COURSE NO LONGER AVAILABLE
The Practical LC/MS On-line Training course covers existing and new approaches to accomplishing on-line LC/MS analyses. The course progresses from an introduction to HPLC and mass spectrometry to themes common to all LC/MS interfaces, emphasizing those phenomena that are important for success with LC/MS experiments. Introductory lectures are followed by more advanced lectures on both atmospheric pressure chemical ionization (APCI) and electrospray.
The content is presented along with representative applications for both small and large molecules, including peptides, proteins, carbohydrates, oligonucleotides, etc. Each lecture includes the “how-to” aspects of each technique with the inclusion of many details and helpful hints for carrying out successful experiments.
Also covered are qualitative approaches to LC/MS, quantitative analysis by LC/MS/MS techniques, the associated GLP aspects of such work, and a detailed overview of current strategies for biological sample preparation for LC/MS analyses. A final summary lecture highlights recent developments in LC/MS including new ionization techniques, mass analyzers, and chip-based devices with selected examples and insights toward the future.